Addgene - hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP

Plasmid 24334: hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

Gene/insert name:
H2B
Species:
D. rerio (zebrafish)
Fusion protein or tag:
GFP
Terminal:
N terminal on insert
Mutation:
mCherry driven by an HSP70 promoter Cerulean driven by a CrystalineA promoter
Vector backbone:
pBluescript
(Search Vector Database)
Vector type:
Worm Expression, Cre/Lox
Backbone size w/o insert (bp):
3000
5' sequencing primer:
SV40pA List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Didier Stainier
Terms and Licenses:
MTA
Clontech Limited Use Label License

Comments:

This plasmid was constructed using 1.5 kb of the hsp70l promoter. The floxed mCherry-STOP and H2b_GFP fragments were PCR amplified individually and sequentially cloned downstream of the hsp70l promoter. The mCherry-STOP cassette serves as a heat-shock induction marker prevents read-through translation of the H2B-GFP fusion protein, which is only expressed in cells that have undergone a Cre mediated excision event. cryaa-Cerulean was inserted downstream of HSB-GFP in the reverse orientation.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Distinct populations of quiescent and proliferative pancreatic beta-cells identified by HOTcre mediated labeling. Hesselson et al (Proc Natl Acad Sci U S A. 2009 Sep 1. 106(35):14896-901. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24334" in your Materials and Methods section.