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hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean
(Plasmid #24334)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24334 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Worm Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B
  • Species
    D. rerio (zebrafish)
  • Mutation
    mCherry driven by an HSP70 promoterCerulean driven by a CrystalineA promoter*
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

Resource Information

Depositor Comments

This plasmid was constructed using 1.5 kb of the hsp70l promoter. The floxed mCherry-STOP and H2b_GFP fragments were PCR amplified individually and sequentially cloned downstream of the hsp70l promoter. The mCherry-STOP cassette serves as a heat-shock induction marker prevents read-through translation of the H2B-GFP fusion protein, which is only expressed in cells that have undergone a Cre mediated excision event. cryaa-Cerulean was inserted downstream of HSB-GFP in the reverse orientation.

*H2B has D26G and V119I mutations. According to the depositor, GFP expression from this construct was always nuclear so there is no reason to suspect that the mutations affect the function (nuclear localization) of the H2B fragment.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean was a gift from Didier Stainier (Addgene plasmid # 24334 ; http://n2t.net/addgene:24334 ; RRID:Addgene_24334)
  • For your References section:

    Distinct populations of quiescent and proliferative pancreatic beta-cells identified by HOTcre mediated labeling. Hesselson D, Anderson RM, Beinat M, Stainier DY. Proc Natl Acad Sci U S A. 2009 Sep 1. 106(35):14896-901. 10.1073/pnas.0906348106 PubMed 19706417