PurposeMammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13777||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4779
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameERT2-Cre-ERT2 fusion protein
Alt nameinducible Cre
Insert Size (bp)2951
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Kozak consensus sequence was added before the start ATG.
The insert can be cut out with EcoRI (SmaI) and NotI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-ERT2CreERT2 was a gift from Connie Cepko (Addgene plasmid # 13777 ; http://n2t.net/addgene:13777 ; RRID:Addgene_13777)
For your References section:Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2007 Jan 5. ():. 10.1073/pnas.0610155104 PubMed 17209010