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pCALNL-DsRed
(Plasmid #13769)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13769 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAGEN
  • Backbone size w/o insert (bp) 6072
  • Vector type
    Mammalian Expression, Cre/Lox ; Flp/FRT
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DsRed2
  • Alt name
    DsRed
  • Alt name
    red fluorescent protein
  • Species
    Discosoma sp
  • Insert Size (bp)
    728

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCAG-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This vector is derived from pCALNL5 (Kanegae et al. NAR 23, 3816-3821 (1995)) obtained from Dr. Saito I (Univ. of Tokyo). DsRed was from pDsRed2-N1 (Clontech).
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

Cre recombinase-dependent inducible expression vector. DsRed is expressed only in the presence of Cre.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCALNL-DsRed was a gift from Connie Cepko (Addgene plasmid # 13769 ; http://n2t.net/addgene:13769 ; RRID:Addgene_13769)
  • For your References section:

    Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2007 Jan 5. ():. 10.1073/pnas.0610155104 PubMed 17209010