Gene/insert name: EGFP
Alt name: GFP
Alt name: green fluorescent protein
Insert size: 772
Species: Aequorea victoria
Vector backbone:
pCAGGS
(Search Vector Database )
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert (bp):
4698
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
pCAG-F
List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
This vector is derived from pCALNL5 (Kanegae et al. NAR 23, 3816-3821 (1995)) obtained from Dr. Saito I (Univ. of Tokyo).
Sequence:
View sequences (2)
Map:
View map
Principal Investigator:
Connie Cepko
Terms and Licenses:
MTA
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
13770" in your Materials and Methods section.
Cre recombinase-dependent inducible expression vector. GFP is expressed only in the presence of Cre.