Addgene - pCAG-Cre:GFP Plasmid Data

Gene/insert name:
Cre-GFP fusion protein
Alt name:
Cre recombinase
Insert size:
1784
Species:
Bacteriophage P1
Fusion protein or tag:
GFP
Terminal:
C terminal on insert
Vector backbone:
pCAGEN
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
4779
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
pCAG-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
The coding sequence of Cre was amplified by PCR using pxCANCre (Kanegae et al. NAR 23, 3816-3821 (1995)) obtained from Dr. Saito I (Univ. of Tokyo) as a template. GFP was from pEGFP-N1 (Clontech).
Sequence: View sequences (4)
Map:
View map
Principal Investigator:
Connie Cepko
Terms and Licenses:
MTA

Comments:

Kozak consensus sequence was added before the start ATG.
GFP-tag was added at the C-terminus of Cre.
Cre-GFP fusion protein is localized in the cell nucleus.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Controlled expression of transgenes introduced by in vivo electroporation. Matsuda et al (Proc Natl Acad Sci U S A. 2007 Jan 5. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13776" in your Materials and Methods section.