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Browse > Connie Cepko > Matsuda et al > pCAG-Cre:GFP
Plasmid 13776: pCAG-Cre:GFP

  • Cre-GFP fusion protein

  • Cre recombinase

  • 1784

  • Bacteriophage P1

  • GFP

  • C terminal on insert

  • pCAGEN
    (Search Vector Database)

  • Mammalian Expression

  • 4779

  • EcoRI

  • No

  • NotI

  • No

  • pCAG-F List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • The coding sequence of Cre was amplified by PCR using pxCANCre (Kanegae et al. NAR 23, 3816-3821 (1995)) obtained from Dr. Saito I (Univ. of Tokyo) as a template. GFP was from pEGFP-N1 (Clontech).

  • View sequences (3)

  • View map

  • Connie Cepko

  • MTA

Comments: 

Kozak consensus sequence was added before the start ATG.
GFP-tag was added at the C-terminus of Cre.
Cre-GFP fusion protein is localized in the cell nucleus.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Controlled expression of transgenes introduced by in vivo electroporation. Matsuda et al (Proc Natl Acad Sci U S A. 2007 Jan 5. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13776" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only