Addgene - pCAG-GFP Plasmid Data

Gene/insert name:
EGFP from Aequorea victoria
Alt name:
GFP
Alt name:
green fluorescent protein
Insert size:
772
Vector backbone:
pCAGEN
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
4779
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
pCAG-F List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
EGFP was from pEGFP-N1 (Clontech).
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Connie Cepko
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Electroporation and RNA interference in the rodent retina in vivo and in vitro. Matsuda et al (Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11150" in your Materials and Methods section.