PurposeMammalian expression vector for expression of DsRed
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11151||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerAvailable at Addgene (#11160)
- Backbone size w/o insert (bp) 4779
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameDsRed2 from Discosoma sp
Alt namered fluorescent protein
Insert Size (bp)728
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCAG-F
- 3′ sequencing primer DsRed-R (Common Sequencing Primers)
CAG promoter (chicken beta-actin promoter with CMV enhancer) is more efficient than CMV promoter, see "Efficient selection for high-expression transfectants with a novel eukaryotic vector", Gene. 1991 Dec 15;108(2):193-9.
Please note that though there are some discrepancies between the assembled sequence from the depositing lab and Addgene's quality control sequence, this plasmid should function as reported.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-DsRed was a gift from Connie Cepko (Addgene plasmid # 11151 ; http://n2t.net/addgene:11151 ; RRID:Addgene_11151)
For your References section:Electroporation and RNA interference in the rodent retina in vivo and in vitro. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. 10.1073/pnas.2235688100 PubMed 14603031