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(Plasmid #11160)


Item Catalog # Description Quantity Price (USD)
Plasmid 11160 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    modified multiple cloning sites
  • Insert Size (bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer pCAG-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pCAGGS (Niwa et al. Gene 108, 193-199 (1991)) was obtained from Dr. J. Miyazaki (Osaka University).
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

The multiple cloning sites of pCAGGS was modified to make pCAGEN (pCAGGS with EcoRI, XhoI, EcoRV and NotI sites).

Note that the Addgene quality control sequence and the author-supplied sequence do not match exactly, but critical features (such as the MCS restriction sites) appear to be intact. If you believe there is something functionally wrong with this plasmid, please report your concern to [email protected]

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGEN was a gift from Connie Cepko (Addgene plasmid # 11160 ; ; RRID:Addgene_11160)
  • For your References section:

    Electroporation and RNA interference in the rodent retina in vivo and in vitro. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. 10.1073/pnas.2235688100 PubMed 14603031