Addgene - pCAGEN Plasmid Data

Gene/insert name:
modified multiple cloning sites
Insert size:
100
Vector backbone:
pCAGGS
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
4698
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
BglII
Site destroyed during cloning:
No
5' sequencing primer:
pCAG-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
pCAGGS (Niwa et al. Gene 108, 193-199 (1991)) was obtained from Dr. J. Miyazaki (Osaka University).
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Connie Cepko
Terms and Licenses:
MTA

Comments:

The multiple cloning sites of pCAGGS was modified to make pCAGEN (pCAGGS with EcoRI, XhoI, EcoRV and NotI sites).

Note that the Addgene quality control sequence and the author-supplied sequence do not match exactly, but critical features (such as the MCS restriction sites) appear to be intact. If you believe there is something functionally wrong with this plasmid, please report your concern to help@addgene.org

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Electroporation and RNA interference in the rodent retina in vivo and in vitro. Matsuda et al (Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11160" in your Materials and Methods section.