Addgene - ins-cre_cryaa-Venus Plasmid Data

Plasmid 24333: ins-cre_cryaa-Venus

Gene/insert name:
Cre
Entrez Gene:
cre (P1_gp003)
Fusion protein or tag:
Venus
Terminal:
C terminal on backbone
Vector backbone:
pBluescript
(Search Vector Database)
Vector type:
Worm Expression, Cre/Lox
Backbone size w/o insert (bp):
3000
5' sequencing primer:
Cre-R List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Didier Stainier
Terms and Licenses:
MTA

Comments:

ins-Cre_cryaa-Venus was generated by placing the Cre coding sequence downstream of 1.5 kb of the proximal insulin promoter. The cryaa-Venus cassette was inserted downstream of Cre in the reverse orientation using 0.54 kb of the cryaa promoter.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Distinct populations of quiescent and proliferative pancreatic beta-cells identified by HOTcre mediated labeling. Hesselson et al (Proc Natl Acad Sci U S A. 2009 Sep 1. 106(35):14896-901. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24333" in your Materials and Methods section.