ins-cre_cryaa-Venus
(Plasmid #24333)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Worm Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    Cre
  • Entrez Gene
    cre (a.k.a. P1_gp003)
  • Tag / Fusion Protein
    • Venus (C terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

ins-Cre_cryaa-Venus was generated by placing the Cre coding sequence downstream of 1.5 kb of the proximal insulin promoter. The cryaa-Venus cassette was inserted downstream of Cre in the reverse orientation using 0.54 kb of the cryaa promoter.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ins-cre_cryaa-Venus was a gift from Didier Stainier (Addgene plasmid # 24333)
  • For your References section:

    Distinct populations of quiescent and proliferative pancreatic beta-cells identified by HOTcre mediated labeling. Hesselson D, Anderson RM, Beinat M, Stainier DY. Proc Natl Acad Sci U S A. 2009 Sep 1. 106(35):14896-901. 10.1073/pnas.0906348106 PubMed 19706417