|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24333||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeWorm Expression, Cre/Lox
Growth in Bacteria
Entrez Genecre (a.k.a. P1_gp003)
/ Fusion Protein
- Venus (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer Cre-R
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
ins-Cre_cryaa-Venus was generated by placing the Cre coding sequence downstream of 1.5 kb of the proximal insulin promoter. The cryaa-Venus cassette was inserted downstream of Cre in the reverse orientation using 0.54 kb of the cryaa promoter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ins-cre_cryaa-Venus was a gift from Didier Stainier (Addgene plasmid # 24333)
For your References section:Distinct populations of quiescent and proliferative pancreatic beta-cells identified by HOTcre mediated labeling. Hesselson D, Anderson RM, Beinat M, Stainier DY. Proc Natl Acad Sci U S A. 2009 Sep 1. 106(35):14896-901. 10.1073/pnas.0906348106 PubMed 19706417