pcDNA3.1 mSTIM1 myc
(Plasmid #17732)

• Depositing Lab: Anjana Rao
• Publication: Oh-Hora et al Nat Immunol. 2008 Apr . 9(4):432-43.

Backbone

• Vector backbone: pcDNA3.1 myc his
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: STIM1
• Species: M. musculus (mouse)
• Entrez Gene: Stim1 (a.k.a. SIM)
• Tag / Fusion Protein:
  • Myc (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: XbaI (destroyed during cloning)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mouse Stim1 (BglII/NotI, NotI is treated by Klenow) was subcloned into BamHI/XbaI (XbaI is treated by Klenow) of pcDNA3.1myc-His vector.