Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21638||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneORAI1 (a.k.a. CRACM1, IMD9, ORAT1, TAM2, TMEM142A)
/ Fusion Proteins
- Myc (C terminal on backbone)
- His (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1 Orai1 was a gift from Anjana Rao (Addgene plasmid # 21638 ; http://n2t.net/addgene:21638 ; RRID:Addgene_21638)
For your References section:Biochemical and functional characterization of Orai proteins. Gwack Y, Srikanth S, Feske S, Cruz-Guilloty F, Oh-hora M, Neems DS, Hogan PG, Rao A. J Biol Chem. 2007 Jun 1. 282(22):16232-43. 10.1074/jbc.M609630200 PubMed 17293345