Addgene - pcDNA3.1 Orai1 Plasmid Data

Plasmid 21638: pcDNA3.1 Orai1

Gene/insert name:
Orai1
Species:
H. sapiens (human)
GenBank ID:
BC015369
Entrez Gene:
ORAI1 (ORAT1, CRACM1, FLJ14466, TMEM142A)
Fusion protein or tag:
Myc
Terminal:
C terminal on backbone
Fusion protein or tag:
His
Terminal:
C terminal on backbone
Vector backbone:
pcDNA3.1/myc-his
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGH rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Principal Investigator:
Anjana Rao
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Biochemical and functional characterization of Orai proteins. Gwack et al (J Biol Chem. 2007 Jun 1. 282(22):16232-43. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21638" in your Materials and Methods section.