pET28a AviTag-DogTag2-MBP
(Plasmid
#201802)
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PurposeBacterial expression of maltose binding protein with a peptide tag for covalent bond formation and an AviTag for site-specific biotinylation.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 201802 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 6636
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMBP
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Alt nameMaltose binding protein
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SpeciesSynthetic
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Insert Size (bp)1317
- Promoter T7
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Tags
/ Fusion Proteins
- AviTag (N terminal on insert)
- DogTag2 (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a AviTag-DogTag2-MBP was a gift from Mark Howarth (Addgene plasmid # 201802) -
For your References section:
Design and Evolution of Enhanced Peptide-Peptide Ligation for Modular Transglutaminase Assembly. Keeble AH, Wood DP, Howarth M. Bioconjug Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122. 10.1021/acs.bioconjchem.3c00122 PubMed 37289810
