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pAZA132
(Plasmid #207645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207645 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pAZA4
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    2 copies of evovled P450-T2 mutant gene and genes for styrene biosynthesis in S. albus
  • Species
    Synthetic
  • Mutation
    P450-T2-5 (S239V F338H P71D T383E)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAZA132 was a gift from Jay Keasling (Addgene plasmid # 207645)

  • For your References section:

    Complete integration of carbene-transfer chemistry into biosynthesis. Huang J, Quest A, Cruz-Morales P, Deng K, Pereira JH, Van Cura D, Kakumanu R, Baidoo EEK, Dan Q, Chen Y, Petzold CJ, Northen TR, Adams PD, Clark DS, Balskus EP, Hartwig JF, Mukhopadhyay A, Keasling JD. Nature. 2023 May;617(7960):403-408. doi: 10.1038/s41586-023-06027-2. Epub 2023 May 3. 10.1038/s41586-023-06027-2 PubMed 37138074
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