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FLAG-EglN2 H358A-pBabe-puro
(Plasmid #22705)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22705 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe-Puro
  • Backbone size w/o insert (bp) 5145
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha or XL1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Egg laying Nine - 2 (EglN2)
  • Alt name
    prolyl hydroxylase 1 (PHD1)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1260
  • Mutation
    mutated cDNA: point mutation in codon 358 changing amino acid H358 to Alanine, rendering the encoded enzyme inactive
  • GenBank ID
    BC036051
  • Entrez Gene
    EGLN2 (a.k.a. EIT-6, EIT6, HIF-PH1, HIFPH1, HPH-1, HPH-3, PHD1)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer pBabe-5'
  • 3′ sequencing primer pBabe-3'
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-EglN2 H358A-pBabe-puro was a gift from William Kaelin (Addgene plasmid # 22705 ; http://n2t.net/addgene:22705 ; RRID:Addgene_22705)
  • For your References section:

    Control of Cyclin D1 and Breast Tumorigenesis by the EglN2 Prolyl Hydroxylase. Zhang Q, Gu J, Li L, Liu J, Luo B, Cheung HW, Boehm JS, Ni M, Geisen C, Root DE, Polyak K, Brown M, Richardson AL, Hahn WC, Kaelin WG, Bommi-Reddy A. Cancer Cell. 2009 Nov 6. 16(5):413-24. 10.1016/j.ccr.2009.09.029 PubMed 19878873