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pCS2 hSmad1-GM
(Plasmid #22992)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22992 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4096
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Smad1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Mutation
    S210A ,T202A, S191A, S198A
  • Entrez Gene
    SMAD1 (a.k.a. BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2 hSmad1-GM was a gift from Edward De Robertis (Addgene plasmid # 22992 ; http://n2t.net/addgene:22992 ; RRID:Addgene_22992)
  • For your References section:

    Integrating patterning signals: Wnt/GSK3 regulates the duration of the BMP/Smad1 signal. Fuentealba LC, Eivers E, Ikeda A, Hurtado C, Kuroda H, Pera EM, De Robertis EM. Cell. 2007 Nov 30. 131(5):980-93. 10.1016/j.cell.2007.09.027 PubMed 18045539