Skip to main content
Addgene
Open Menu

pUC19BAblue-Ki_gcd1
(Plasmid #246236)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246236 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pUC19

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Cytosine permease
  • Alt name
    codB
  • Species
    E. coli BL21 (TaKaRa)
  • Insert Size (bp)
    1260
  • GenBank ID
    CP010816.1
  • Promoter J23104

Cloning Information for Gene/Insert 1

  • Cloning method Other
  • 5′ sequencing primer gtgtcgcaagataacaactttagccag
  • 3′ sequencing primer tcaacgtttgtaatcgatggcttctg
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Cytosine deaminase
  • Alt name
    codA
  • Species
    E. coli BL21 (TaKaRa)
  • Insert Size (bp)
    1289
  • Mutation
    See depositor comments below.
  • GenBank ID
    CP010816.1
  • Promoter J23104

Cloning Information for Gene/Insert 2

  • Cloning method Other
  • 5′ sequencing primer gtgtcgcaagataacaactttagccag
  • 3′ sequencing primer tcaacgtttgtaatcgatggcttctg
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    Indigoidine synthase
  • Alt name
    bpsA
  • Species
    Streptomyces lavendulae subsp. lavendulae NBRC 12340
  • Insert Size (bp)
    3841
  • Mutation
    See depositor comments below.
  • GenBank ID
    AB240063
  • Promoter Trc promoter

Cloning Information for Gene/Insert 3

  • Cloning method Other
  • 5′ sequencing primer ggatccACGGAGGAGGCAGAATTCatgactcttcaggagaccagcg
  • 3′ sequencing primer tcctctgttctagattcactcgccgagcaggtagc
  • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    4'-phosphopantetheinyl transferase
  • Alt name
    pptA
  • Species
    Streptomyces mobaraensis NBRC 13819
  • Insert Size (bp)
    738
  • GenBank ID
    PRJDB448
  • Promoter Trc promoter

Cloning Information for Gene/Insert 4

  • Cloning method Other
  • 5′ sequencing primer ATCTAGAACAGAGGAAGCAGAATTCatcgccgccctcctgc
  • 3′ sequencing primer ttacgggacggcggtcc
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains a Q419stop mutation in codA and a Q335P mutation in bpsA. These mutations are not known to impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19BAblue-Ki_gcd1 was a gift from Kenji Tajima (Addgene plasmid # 246236 ; http://n2t.net/addgene:246236 ; RRID:Addgene_246236)

  • For your References section:

    Enhanced production of nanofibrillated bacterial cellulose through regulation of gluconic acid metabolism. Takahama R, Takayama G, Suginaka M, Ishido Y, Nagai S, Nagai K, Uenishi M, Tajima K. Appl Environ Microbiol. 2026 Oct 7:e0170725. doi: 10.1128/aem.01707-25. 10.1128/aem.01707-25 PubMed 42841632
Related items: