pCDNA3.1(+) GFP-PIPKI{gamma}661-FLAG
(Plasmid
#29583)
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Depositing Lab
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Depositing OrganizationUniversity of Wisconsin, Madison
Located in United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 29583 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 29583-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 29583-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCDNA3.1(+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5424
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5 alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namephosphatidylinositol phosphate kinase type I gamma
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Alt namePIPKI gamma 661
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SpeciesM. musculus (mouse)
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Insert Size (bp)2715
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MutationN/A
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Tags
/ Fusion Proteins
- GFP (N terminal on insert)
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byLing et al., 2002 from Dr. Richard Anderson
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp#440 when compared to the full sequence information provided by the depositing laboratory.
DNA (Catalog # 29583-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 29583-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3.1(+) GFP-PIPKI{gamma}661-FLAG was a gift from Anna Huttenlocher (Addgene plasmid # 29583 ; http://n2t.net/addgene:29583 ; RRID:Addgene_29583) -
For your References section:
Type Igamma PIP kinase is a novel uropod component that regulates rear retraction during neutrophil chemotaxis. Lokuta MA, Senetar MA, Bennin DA, Nuzzi PA, Chan KT, Ott VL, Huttenlocher A. Mol Biol Cell. 2007 Dec . 18(12):5069-80. 10.1091/mbc.e07-05-0428 PubMed 17928408
