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Plasmid 29584: mCherryC1-PIPKI{gamma}661
  • phosphatidylinositol phosphate kinase type I gamma

  • PIPKI gamma 661

  • 1986

  • M. musculus (mouse)

  • mCherry

  • N terminal on insert

  • mCherry C1
    (Search Vector Database)

  • Mammalian Expression

  • 4673

  • Bgl II

  • No

  • Kpn1

  • No

  • n/a List of Sequencing Primers

  • Kanamycin

  • DH5alpha

  • 37

  • DH5 alpha

  • High Copy

  • Ling et al., 2002 gift from Dr. Richard Anderson

  • View sequences (2)
  • Anna Huttenlocher

  • UBMTA
    Clontech Limited Use Label License
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

pmCherry-C1 was generated by PCR amplification of pRSET-B-mCherry (a generous gift from Dr. Roger Tsien) with primers containing a 5' NheI site and 3' BglII site and subcloning into pEGFP-C1 (Clontech) to replace the enhanced GFP (EGFP) sequence

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Type Igamma PIP kinase is a novel uropod component that regulates rear retraction during neutrophil chemotaxis. Lokuta et al (Mol Biol Cell. 2007 Dec . 18(12):5069-80. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 29584" in your Materials and Methods section.