mCherryC1-PIPKI{gamma}661
(Plasmid #29584)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    mCherry C1
  • Backbone size w/o insert (bp) 4673
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    phosphatidylinositol phosphate kinase type I gamma
  • Alt name
    PIPKI gamma 661
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1986
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (not destroyed)
  • 3′ cloning site Kpn1 (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

pmCherry-C1 was generated by PCR amplification of pRSET-B-mCherry (a generous gift from Dr. Roger Tsien) with primers containing a 5' NheI site and 3' BglII site and subcloning into pEGFP-C1 (Clontech) to replace the enhanced GFP (EGFP) sequence

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherryC1-PIPKI{gamma}661 was a gift from Anna Huttenlocher (Addgene plasmid # 29584)
  • For your References section:

    Type Igamma PIP kinase is a novel uropod component that regulates rear retraction during neutrophil chemotaxis. Lokuta MA, Senetar MA, Bennin DA, Nuzzi PA, Chan KT, Ott VL, Huttenlocher A. Mol Biol Cell. 2007 Dec . 18(12):5069-80. 10.1091/mbc.E07-05-0428 PubMed 17928408