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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 13763 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTriEx-1
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5612
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Vector typeBacterial Expression ; Baculovirus
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTAT-NLS-Cre
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Alt nameTAT-Cre
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Alt nameHTNC
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Alt nameHTNCre
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SpeciesNLS from SV40, TAT from HIV, and Cre from bacteriophage P1
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Insert Size (bp)932
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Tags
/ Fusion Proteins
- His Tag (N terminal on insert)
- TAT (N terminal on insert)
- NLS (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
Depositor Comments
Please also acknowledge Dr. Frank Edenhofer when using this plasmid in publications.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTriEx-HTNC was a gift from Klaus Rajewsky (Addgene plasmid # 13763 ; http://n2t.net/addgene:13763 ; RRID:Addgene_13763) -
For your References section:
Ability of the hydrophobic FGF and basic TAT peptides to promote cellular uptake of recombinant Cre recombinase: a tool for efficient genetic engineering of mammalian genomes. Peitz M, Pfannkuche K, Rajewsky K, Edenhofer F. Proc Natl Acad Sci U S A. 2002 Apr 2. 99(7):4489-94. 10.1073/pnas.032068699 PubMed 11904364