pTriEx-HTNC
(Plasmid #13763)

• Depositing Lab: Klaus Rajewsky
• Publication: Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99(7):4489-94.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	13763	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pTriEx-1
  (Search Vector Database)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5612
• Vector type: Bacterial Expression ; Baculovirus

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TAT-NLS-Cre
• Alt name: TAT-Cre
• Alt name: HTNC
• Alt name: HTNCre
• Species: NLS from SV40, TAT from HIV, and Cre from bacteriophage P1
• Insert Size (bp): 932
• Tags / Fusion Proteins:
  • His Tag (N terminal on insert)
  • TAT (N terminal on insert)
  • NLS (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Terms and Licenses:
  • UBMTA
  • EMD Millipore Limited Use Label License pTriEx Assurance Letter
• Articles Citing this Plasmid:
  • 10 References

Depositor Comments

Please also acknowledge Dr. Frank Edenhofer when using this plasmid in publications.