pTriEx-HTNC
(Plasmid #13763)

• Purpose: For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.
• Depositing Lab: Klaus Rajewsky
• Publication: Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99(7):4489-94.

Backbone

• Vector backbone: pTriEx-1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5612
• Vector type: Bacterial Expression ; Baculovirus

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: His-TAT-NLS-Cre
• Alt name: TAT-Cre
• Alt name: HTNC
• Alt name: HTNCre
• Species: NLS from SV40, TAT from HIV, and Cre from bacteriophage P1
• Insert Size (bp): 932
• Promoter: p10 promoter
• Tags / Fusion Proteins:
  • His Tag (N terminal on insert)
  • TAT (N terminal on insert)
  • NLS (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please also acknowledge Dr. Frank Edenhofer when using this plasmid in publications.