Addgene - Lox-Stop-Lox TOPO Plasmid Data

Plasmid 11584: Lox-Stop-Lox TOPO

Gene/insert name:
loxP-STOP-loxP
Insert size:
5700
Species:
SV40
Vector backbone:
pCR-Blunt II-TOPO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert (bp):
3500
Cloning method:
TOPO Cloning
5' sequencing primer:
M13R List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
Stbl3
Growth temperature (℃):
30
Growth instructions:
However, plasmid yield and stability is improved when grown at 30 degrees.
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (4)
Map:
View map
Supplemental document:
LSL-info (application/pdf)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

Plasmid containing LoxP-STOP-LoxP cassette for use in expressing genes only after cre-mediated recombination.

Please also see Lox-Stop-Lox TOPO Hygro (http://www.addgene.org/35688) for a Hygromycin version of this plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Analysis of lung tumor initiation and progression using conditional expression of oncogenic K-ras. Jackson et al (Genes Dev. 2001 Dec 15. 15(24):3243-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11584" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

13777: pCAG-ERT2CreERT2