|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11584||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepCR-Blunt II-TOPO
- Backbone size w/o insert (bp) 3500
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Growth instructionsHowever, plasmid yield and stability is improved when grown at 30 degrees.
Copy numberHigh Copy
Insert Size (bp)5700
- Cloning method TOPO Cloning
- 5′ sequencing primer M13R (Common Sequencing Primers)
Plasmid containing LoxP-STOP-LoxP cassette for use in expressing genes only after cre-mediated recombination.
Please also see Lox-Stop-Lox TOPO Hygro (http://www.addgene.org/35688) for a Hygromycin version of this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Lox-Stop-Lox TOPO was a gift from Tyler Jacks (Addgene plasmid # 11584)
For your References section:Analysis of lung tumor initiation and progression using conditional expression of oncogenic K-ras. Jackson EL, Willis N, Mercer K, Bronson RT, Crowley D, Montoya R, Jacks T, Tuveson DA. Genes Dev. 2001 Dec 15. 15(24):3243-8. 10.1101/gad.943001 PubMed 11751630
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.