Lox-Stop-Lox TOPO Hygro
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35688||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepCR-Blunt II-TOPO
- Backbone size w/o insert (bp) 3500
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Growth instructionsPlasmid yield and stability is improved when grown at 30 degrees.
Copy numberHigh Copy
Alt nameloxP STOP loxP
Insert Size (bp)6100
- Cloning method TOPO Cloning
- 5′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
If you are using the cassette for gene targeting, you should check the number of SV40 repeats in the targeted locus as the lab has noticed that in some ES clones copies of the repeats were missing.
Please also see Lox-Stop-Lox TOPO (http://www.addgene.org/11584/) for a puromycin version of this plasmid and to download a document about using the Lox-Stop-Lox system.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Lox-Stop-Lox TOPO Hygro was a gift from Jacqueline Lees (Addgene plasmid # 35688 ; http://n2t.net/addgene:35688 ; RRID:Addgene_35688)