Addgene - Lox-Stop-Lox TOPO Hygro Plasmid Data

Plasmid 35688: Lox-Stop-Lox TOPO Hygro

Gene/insert name:
loxP-STOP-loxP
Alt name:
loxP STOP loxP
Insert size:
6100
Species:
SV40
Vector backbone:
pCR-Blunt II-TOPO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert (bp):
3500
Cloning method:
TOPO Cloning
5' sequencing primer:
M13R List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
Stbl3
Growth temperature (℃):
30
Growth instructions:
Plasmid yield and stability is improved when grown at 30 degrees.
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (4)
Principal Investigator:
Jacqueline Lees
Terms and Licenses:
MTA

Comments:

If you are using the cassette for gene targeting, you should check the number of SV40 repeats in the targeted locus as the lab has noticed that in some ES clones copies of the repeats were missing.

Please also see Lox-Stop-Lox TOPO (http://www.addgene.org/11584/) for a puromycin version of this plasmid and to download a document about using the Lox-Stop-Lox system.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35688" in your Materials and Methods section.