Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.
( Search Vector Database)
Mammalian Expression, Retroviral, Cre/Lox
Backbone size w/o insert (bp):
Modifications to Backbone:
loxp-dsRed-loxp-MCS-eGFP seq is inserted.
Ligation Independent Cloning
5' sequencing primer:
List of Sequencing Primers
Growth temperature (℃):
The depositor notes that XL1-Blue is also ok for general purpose use.
High or low copy:
View sequences (4)
In fusion cloning in pMSCV-loxp-dsRed-loxp vectors (application/pdf)
Terms and Licenses:
Clontech Limited Use Label License
Addgene has sequenced a portion of this plasmid for verification.
here for the sequencing
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
32702" in your Materials and Methods section.