Addgene - pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE Plasmid Data

Plasmid 32702: pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

Gene/insert name:
eGFP
Insert size:
720
Vector backbone:
MSCV
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression, Retroviral, Cre/Lox
Backbone size w/o insert (bp):
6295
Modifications to Backbone:
loxp-dsRed-loxp-MCS-eGFP seq is inserted.
Cloning method:
Ligation Independent Cloning
5' sequencing primer:
CTCCCACAACGAGGACTACAC List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
The depositor notes that XL1-Blue is also ok for general purpose use.
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (3)
Map:
View map
Supplemental document:
In fusion cloning in pMSCV-loxp-dsRed-loxp vectors (application/pdf)
Principal Investigator:
Hans Clevers
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Controlled gene expression in primary Lgr5 organoid cultures. Koo et al (Nat Methods. 2011 Dec 4. doi: 10.1038/nmeth.1802. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32702" in your Materials and Methods section.