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pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE
(Plasmid #32702)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    MSCV
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6295
  • Modifications to backbone
    loxp-dsRed-loxp-MCS-eGFP seq is inserted.
  • Vector type
    Mammalian Expression, Retroviral, Cre/Lox
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    The depositor notes that XL1-Blue is also ok for general purpose use.
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    eGFP
  • Insert Size (bp)
    720

Cloning Information

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE was a gift from Hans Clevers (Addgene plasmid # 32702)
  • For your References section:

    Controlled gene expression in primary Lgr5 organoid cultures. Koo BK, Stange DE, Sato T, Karthaus W, Farin HF, Huch M, van Es JH, Clevers H. Nat Methods. 2011 Dec 4. doi: 10.1038/nmeth.1802. 10.1038/nmeth.1802 PubMed 22138822