It is a vector for cloning new miRNA.
( Search Vector Database)
Richard O. Hynes group
Mammalian Expression, Retroviral, RNAi, Cre/Lox
Modifications to Backbone:
1) GFP to dsRed
2) Thy1.1-mir30 to emGFP-miR (Invitrogen)
Ligation Independent Cloning
5' sequencing primer:
List of Sequencing Primers
Growth temperature (℃):
The depositor notes that XL1-Blue is also ok for general purpose use.
High or low copy:
View sequences (4)
Terms and Licenses:
Clontech Limited Use Label License
Addgene has sequenced a portion of this plasmid for verification.
here for the sequencing
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
32704" in your Materials and Methods section.