Addgene - pMSCV-FLIP-puro-dsRed-GFP-miRNA Plasmid Data

Plasmid 32704: pMSCV-FLIP-puro-dsRed-GFP-miRNA

Gene/insert name:
emGFP-miRNA
shRNA sequence:
It is a vector for cloning new miRNA.
Vector backbone:
MSCV
(Search Vector Database)
Backbone manufacturer:
Richard O. Hynes group
Vector type:
Mammalian Expression, Retroviral, RNAi, Cre/Lox
Modifications to Backbone:
1) GFP to dsRed 2) Thy1.1-mir30 to emGFP-miR (Invitrogen)
Cloning method:
Ligation Independent Cloning
5' sequencing primer:
CTCCCACAACGAGGACTACAC List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
The depositor notes that XL1-Blue is also ok for general purpose use.
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (4)
Map:
View map
Principal Investigator:
Hans Clevers
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Controlled gene expression in primary Lgr5 organoid cultures. Koo et al (Nat Methods. 2011 Dec 4. doi: 10.1038/nmeth.1802. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32704" in your Materials and Methods section.