Addgene - MSCV CreERT2 puro Plasmid Data

Plasmid 22776: MSCV CreERT2 puro

Gene/insert name:
CreERT2
Alt name:
Cre-ER-T2
Alt name:
Cre-ERT2
Vector backbone:
pMSCV puro
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
6300
5' sequencing primer:
MSCV List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (2)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

Cre-ERT2 contains Cre fused to the ligand-binding domain of a mutated estrogen receptor, which recognizes tamoxifen or its derivative 4-hydroxytamoxifen(4-OHT). This construct permits tamoxifen-dependent Cre activity.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Dicer1 functions as a haploinsufficient tumor suppressor. Kumar et al (Genes Dev. 2009 Nov 10. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22776" in your Materials and Methods section.