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Plasmid 17408: Puro.Cre empty vector
  • None

  • Puro.Cre
    (Search Vector Database)

  • Jacks Lab

  • Mammalian Expression, Lentiviral, Cre/Lox

  • 9200

  • n/a List of Sequencing Primers

  • MSCV rev

  • Ampicillin

  • Stbl3

  • 37

  • Unknown

  • Puromycin

  • UbC.Luciferase.PGK.Cre was a kind gift of M. Dupage

  • View sequences (2)
  • Tyler Jacks

  • UBMTA

Comments: 

Puro.Cre was initially generated by amplification of PuroR from MSCV.Puro (Clontech) with PuroF (5’-CGCTGCGCCGAATTCATGACCGAGTACAAGCCCACG-3’) and PuroR (5’-TAAGCGGCCGCTCGAGTCAGGCACCGGGCTTGCGGGTC-3’), digestion with EcoRI and NotI and cloning into UbC.Luciferase.PGK.Cre to generate Puro.Cre.

For lentiviral packaging, you can use pCMV-delta-8.2 (Addgene #8455) and pCMV-VSV-G (Addgene #8454).

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Suppression of non-small cell lung tumor development by the let-7 microRNA family. Kumar et al (Proc Natl Acad Sci U S A. 2008 Feb 28. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17408" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only