Addgene - Puro.Cre empty vector Plasmid Data

Plasmid 17408: Puro.Cre empty vector

Gene/insert name:
None
Vector backbone:
Puro.Cre
(Search Vector Database)
Backbone manufacturer:
Jacks Lab
Vector type:
Mammalian Expression, Lentiviral, Cre/Lox
Backbone size (bp):
9200
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
MSCV rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
UbC.Luciferase.PGK.Cre was a kind gift of M. Dupage
Sequence: View sequences (2)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

Puro.Cre was initially generated by amplification of PuroR from MSCV.Puro (Clontech) with PuroF (5’-CGCTGCGCCGAATTCATGACCGAGTACAAGCCCACG-3’) and PuroR (5’-TAAGCGGCCGCTCGAGTCAGGCACCGGGCTTGCGGGTC-3’), digestion with EcoRI and NotI and cloning into UbC.Luciferase.PGK.Cre to generate Puro.Cre.

For lentiviral packaging, you can use pCMV-delta-8.2 (Addgene #8455) and pCMV-VSV-G (Addgene #8454).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Suppression of non-small cell lung tumor development by the let-7 microRNA family. Kumar et al (Proc Natl Acad Sci U S A. 2008 Feb 28. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17408" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

20781: GFP.Cre empty vector

8455: pCMV-dR8.2 dvpr

8454: pCMV-VSV-G

11586: pSico PGK puro

17409: Puro.let-7g.Cre