p079 pPNT6
(Plasmid #11072)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pPNT6
  • Backbone size (bp) 7500
  • Vector type
    Mammalian Expression, Cre/Lox
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Targeting vector with Neo flanked by 2 LoxP sequences. Also called pPNT3LoxP2. Ligation of Neo Cassette from pPNT3.LoxP5' cut AscI/XbaI into pPNT3.LoxP3' cut AscI/XbaI. The resulting plasmid has Neomycin Cassette flanked with 2 LoxP sequences oriented in the same direction.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p079 pPNT6 was a gift from Ramesh Shivdasani (Addgene plasmid # 11072)