- Backbone size (bp) 7500
Vector typeMammalian Expression, Cre/Lox
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Targeting vector with Neo flanked by 2 LoxP sequences. Also called pPNT3LoxP2. Ligation of Neo Cassette from pPNT3.LoxP5' cut AscI/XbaI into pPNT3.LoxP3' cut AscI/XbaI. The resulting plasmid has Neomycin Cassette flanked with 2 LoxP sequences oriented in the same direction.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p079 pPNT6 was a gift from Ramesh Shivdasani (Addgene plasmid # 11072)
Map generated by Addgene from full sequence supplied by depositor.