Addgene - p079 pPNT6 Plasmid Data

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Plasmid 11072: p079 pPNT6

Gene/insert name:
None
Vector backbone:
pPNT6
(Search Vector Database)
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert:
7500
5' sequencing primer:
M13R List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Ramesh Shivdasani
Terms and Licenses:
MTA

Comments:

Targeting vector with Neo flanked by 2 LoxP sequences. Also called pPNT3LoxP2. Ligation of Neo Cassette from pPNT3.LoxP5' cut AscI/XbaI into pPNT3.LoxP3' cut AscI/XbaI. The resulting plasmid has Neomycin Cassette flanked with 2 LoxP sequences oriented in the same direction.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11072" in your Materials and Methods section.