|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11072||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7500
Vector typeMammalian Expression, Cre/Lox
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
Targeting vector with Neo flanked by 2 LoxP sequences. Also called pPNT3LoxP2. Ligation of Neo Cassette from pPNT3.LoxP5' cut AscI/XbaI into pPNT3.LoxP3' cut AscI/XbaI. The resulting plasmid has Neomycin Cassette flanked with 2 LoxP sequences oriented in the same direction.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p079 pPNT6 was a gift from Ramesh Shivdasani (Addgene plasmid # 11072 ; http://n2t.net/addgene:11072 ; RRID:Addgene_11072)