|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22799||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneRosa26 Targeting Vector
- Backbone size w/o insert (bp) 9862
Vector typeMouse Targeting
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Growth instructionsBest grown in bacterial strain that minimizes recombination (i.e., STBL2).
Copy numberHigh Copy
Gene/Insert nameCAG-Floxed tdTomato
Insert Size (bp)7057
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Ai9 was a gift from Hongkui Zeng (Addgene plasmid # 22799 ; http://n2t.net/addgene:22799 ; RRID:Addgene_22799)
For your References section:A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H. Nat Neurosci. 2010 Jan . 13(1):133-40. 10.1038/nn.2467 PubMed 20023653