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pROSA26-1
(Plasmid #21714)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21714 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript KS (-)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4486
  • Vector type
    Mouse Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ROSA26
  • Alt name
    gene trap ROSA26
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    5360
  • Entrez Gene
    Gt(ROSA)26Sor (a.k.a. AV258896, Gtrgeo26, Gtrosa26, R26, ROSA26, Thumpd3as1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

General ROSA26-1 targeting vector. For more information visit - http://research.mssm.edu/soriano/lab

We recommend you order this plasmid along with SAbgeo and pROSA26-5' as a "ROSA26 targeting kit".

Please note that although the full plasmid sequence indicates PmeI does not cut the plasmid, there is a PmeI site somewhere in the plasmid. See Notes from Addgene link above for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pROSA26-1 was a gift from Philippe Soriano (Addgene plasmid # 21714 ; http://n2t.net/addgene:21714 ; RRID:Addgene_21714)
  • For your References section:

    Generalized lacZ expression with the ROSA26 Cre reporter strain. Soriano P.. Nat Genet. 1999 Jan;21(1):70-1. 10.1038/5007 PubMed 9916792