|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21714||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript KS (-)
- Backbone size w/o insert (bp) 4486
Vector typeMouse Targeting
Growth in Bacteria
Copy numberHigh Copy
Alt namegene trap ROSA26
SpeciesM. musculus (mouse)
Insert Size (bp)5360
Entrez GeneGt(ROSA)26Sor (a.k.a. AV258896, Gtrgeo, Gtrgeo26, Gtro, Gtrosa26, R2, R26, RO, ROSA26, Thumpd3, Thumpd3as1)
- Cloning method Restriction Enzyme
- 5′ cloning site SacII (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
General ROSA26-1 targeting vector. For more information visit - http://research.mssm.edu/soriano/lab
We recommend you order this plasmid along with SAbgeo and pROSA26-5' as a "ROSA26 targeting kit".
Please note that although the full plasmid sequence indicates PmeI does not cut the plasmid, there is a PmeI site somewhere in the plasmid. See Notes from Addgene link above for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pROSA26-1 was a gift from Philippe Soriano (Addgene plasmid # 21714 ; http://n2t.net/addgene:21714 ; RRID:Addgene_21714)
For your References section:Generalized lacZ expression with the ROSA26 Cre reporter strain. Soriano P.. Nat Genet. 1999 Jan;21(1):70-1. 10.1038/5007 PubMed 9916792