|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21715||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBluescript KS (-)
- Backbone size w/o insert (bp) 3027
Vector typefor use with mouse targeting vector pROSA26-1
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameROSA26-5' insert
SpeciesM. musculus (mouse)
Insert Size (bp)132
Entrez GeneGt(ROSA)26Sor (a.k.a. AV258896, Gtrgeo26, Gtrosa26, R26, ROSA26, Thumpd3as1)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Probe for detecting targeted integrations by Southern blots. It is a small probe so users should wash at low stringency, eg. 1xSSC at 65 degrees.
We recommend you order this plasmid along with pROSA26-1 and SAbgeo as a "ROSA26 targeting kit".
For more information visit -
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pROSA26-5' was a gift from Philippe Soriano (Addgene plasmid # 21715 ; http://n2t.net/addgene:21715 ; RRID:Addgene_21715)
For your References section:Generalized lacZ expression with the ROSA26 Cre reporter strain. Soriano P.. Nat Genet. 1999 Jan;21(1):70-1. 10.1038/5007 PubMed 9916792