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Plasmid
21270:
pBigT
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Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. Srinivas et al (BMC Dev Biol. 2001 . 1():4. PubMed) Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21270" in your Materials and Methods section. |
pBigT is used for making transgenic mice. The plasmid contains a loxP-flanked cassette with a PGK-neo selectable
marker and a tpA transcriptional stop sequence. See article for detailed information regarding the creation and use of this plasmid.