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Plasmid 21270: pBigT
  • None

  • pBigT
    (Search Vector Database)

  • Costantini Lab

  • Mouse Targeting

  • 6117

  • n/a List of Sequencing Primers

  • BGH reverse

  • Ampicillin

  • DH5alpha

  • 37

  • Unknown

  • Neomycin

  • View sequences (2)
  • View map

  • Frank Costantini

  • MTA

Comments: 

pBigT is used for making transgenic mice. The plasmid contains a loxP-flanked cassette with a PGK-neo selectable
marker and a tpA transcriptional stop sequence. See article for detailed information regarding the creation and use of this plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. Srinivas et al (BMC Dev Biol. 2001 . 1():4. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21270" in your Materials and Methods section.