pBTG (pBigT-IRES-GFP; DM#268)
(Plasmid #15037)

• Purpose: (Empty Backbone)
• Depositing Lab: Douglas Melton
• Publication: Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 100(25):14920-5.

Backbone

• Vector backbone: pBigT
• Backbone size (bp): 7562
• Vector type: Cre/Lox ; for making transgenic constructs
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • IRES-EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pBigT from Frank Costantini.
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Modification of plasmid pBigT described in Srinivas et al. BMC Dev Biol (2001) vol 1, p.4. For use with pRosa26PA. See BMC Dev Biol article for more information.

Modified to contain IRES-EGFP downstream of cloning site. See Author's Map for more information.