pBTG (pBigT-IRES-GFP; DM#268)
(Plasmid #15037)

• Purpose: (Empty Backbone)
• Depositing Lab: Douglas Melton
• Publication: Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 100(25):14920-5.
• Sequence Information:

  • Depositor Sequences: None.
  • Addgene Sequences: Partial (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	15037	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pBigT
  (Search Vector Database)
• Backbone size (bp): 7562
• Vector type: Cre/Lox ; for making transgenic constructs
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • IRES-EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pBigT from Frank Costantini.
• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Articles Citing this Plasmid:
  • 4 References

Depositor Comments

Modification of plasmid pBigT described in Srinivas et al. BMC Dev Biol (2001) vol 1, p.4. For use with pRosa26PA. See BMC Dev Biol article for more information.

Modified to contain IRES-EGFP downstream of cloning site. See Author's Map for more information.