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pROSA26-PA
(Plasmid #21271)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21271 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pROSA26-1
  • Backbone manufacturer
    Available at Addgene (Plasmid# 21714)
  • Vector type
    Mouse Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ROSA26
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Gt(ROSA)26Sor (a.k.a. AV258896, Gtrgeo26, Gtrosa26, R26, ROSA26, Thumpd3as1)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Philippe M. Soriano provided pROSA26-1.
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

To make the ROSA26 genomic sequence compatible with the pBigT plasmid, pROSA26-1 was digested with XbaI, Klenow filled and a linker (PacI, SwaI, AscI) inserted. This plasmid was called pROSA26PA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pROSA26-PA was a gift from Frank Costantini (Addgene plasmid # 21271 ; http://n2t.net/addgene:21271 ; RRID:Addgene_21271)
  • For your References section:

    Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. Srinivas S, Watanabe T, Lin CS, William CM, Tanabe Y, Jessell TM, Costantini F. BMC Dev Biol. 2001 . 1():4. 10.1186/1471-213X-1-4 PubMed 11299042