|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21271||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAvailable at Addgene (Plasmid# 21714)
Vector typeMouse Targeting
Growth in Bacteria
SpeciesM. musculus (mouse)
Entrez GeneGt(ROSA)26Sor (a.k.a. AV258896, Gtrgeo26, Gtrosa26, R26, ROSA26, Thumpd3as1)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
To make the ROSA26 genomic sequence compatible with the pBigT plasmid, pROSA26-1 was digested with XbaI, Klenow filled and a linker (PacI, SwaI, AscI) inserted. This plasmid was called pROSA26PA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pROSA26-PA was a gift from Frank Costantini (Addgene plasmid # 21271 ; http://n2t.net/addgene:21271 ; RRID:Addgene_21271)
For your References section:Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. Srinivas S, Watanabe T, Lin CS, William CM, Tanabe Y, Jessell TM, Costantini F. BMC Dev Biol. 2001 . 1():4. 10.1186/1471-213X-1-4 PubMed 11299042