|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21710||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript KS (-)
- Backbone size w/o insert (bp) 2911
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameROSA26 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)813
Entrez GeneGt(ROSA)26Sor (a.k.a. AV258896, Gtrgeo, Gtrgeo26, Gtro, Gtrosa26, R2, R26, RO, ROSA26, Thumpd3, Thumpd3as1)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Fragment 5' of exon 1; promoter fragment for driving expression.
For more information visit -
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pROSA26-promoter was a gift from Philippe Soriano (Addgene plasmid # 21710 ; http://n2t.net/addgene:21710 ; RRID:Addgene_21710)
For your References section:Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells. Zambrowicz BP, Imamoto A, Fiering S, Herzenberg LA, Kerr WG, Soriano P. Proc Natl Acad Sci U S A. 1997 Apr 15. 94(8):3789-94. 10.1073/pnas.94.8.3789 PubMed 9108056