Addgene - STOP-eGFP-ROSA26TV Plasmid Data

Plasmid 11739: STOP-eGFP-ROSA26TV

Gene/insert name:
STOP-eGFP-ROSA26TV
Insert size:
11500
Species:
DNA derived from yeast E. Coli, transposon Tn5, Corynebacterium Aequoria (jellyfish)
Vector backbone:
pBSII KS
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Cre/Lox ; /STOP
Backbone size w/o insert (bp):
2900
Cloning site 5':
SnaBI
Site destroyed during cloning:
No
Cloning site 3':
SacI
Site destroyed during cloning:
No
5' sequencing primer:
T7/M13 List of Sequencing Primers
3' sequencing primer:
T7/M13
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Selectable markers, other:
Diptheriatoxin A
Person or lab that originally
cloned the gene/insert:
Based on pRosa26-1 from Soriano P, Fred Hutchinson, Cancer Research Institute
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Klaus Rajewsky
Terms and Licenses:
MTA

Comments:

Targeting vector for cDNA knock-in into the Rosa26 locus.

See more information about the creation of this plasmid on the PDF attached to the CTV vector page http://www.addgene.org/15912

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Canonical NF-kappaB activity, dispensable for B cell development, replaces BAFF-receptor signals and promotes B cell proliferation upon activation. Sasaki et al (Immunity. 2006 Jun . 24(6):729-39. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11739" in your Materials and Methods section.