|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11739||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBSII KS
- Backbone size w/o insert (bp) 2900
Vector typeCre/Lox ; /STOP
Selectable markersNeomycin (select with G418) ; Diptheriatoxin A
Growth in Bacteria
Copy numberHigh Copy
SpeciesDNA derived from yeast E. Coli, transposon Tn5, Corynebacterium Aequoria (jellyfish)
Insert Size (bp)11500
- Cloning method Restriction Enzyme
- 5′ cloning site SnaBI (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer T7/M13
- 3′ sequencing primer T7/M13 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byBased on pRosa26-1 from Soriano P, Fred Hutchinson, Cancer Research Institute
Terms and Licenses
Articles Citing this Plasmid
Targeting vector for cDNA knock-in into the Rosa26 locus.
See more information about the creation of this plasmid on the PDF attached to the CTV vector page http://www.addgene.org/15912
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:STOP-eGFP-ROSA26TV was a gift from Klaus Rajewsky (Addgene plasmid # 11739 ; http://n2t.net/addgene:11739 ; RRID:Addgene_11739)
For your References section:Canonical NF-kappaB activity, dispensable for B cell development, replaces BAFF-receptor signals and promotes B cell proliferation upon activation. Sasaki Y, Derudder E, Hobeika E, Pelanda R, Reth M, Rajewsky K, Schmidt-Supprian M. Immunity. 2006 Jun . 24(6):729-39. 10.1016/j.immuni.2006.04.005 PubMed 16782029