|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15912||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBSII KS
- Backbone size w/o insert (bp) 2900
Vector typeCre/Lox ; /STOP
Selectable markersNeomycin (select with G418) ; Diptheriatoxin A
Growth in Bacteria
Growth instructionsDue to the large size of this plasmid, it takes longer to grow the bacteria to obtain sufficient amount of DNA to work with. I normally grow the bacteria at 37C in LB for 24-36 hours before extracting the plasmid.
Copy numberHigh Copy
SpeciesDNA derived from yeast, E. coli transposon Tn5 Corynebacterium aequoria (jellyfish)
Insert Size (bp)16103
- Cloning method Restriction Enzyme
- 5′ cloning site SnaBI (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer T7/M13
- 3′ sequencing primer T7/M13 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byBased on STOP-eGFP-ROSA26TV by Klaus Rajewsky, IDI, Immune Disease Institute.
Terms and Licenses
Articles Citing this Plasmid
See author's map and attached PDF for more information.
The plasmid is also used in: Xiao C, Calado DP, Galler G, Thai TH, Patterson HC, Wang J, Rajewsky N, Bender TP, Rajewsky K. MiR-150 Controls B Cell Differentiation by Targeting the Transcription Factor c-Myb.
Cell. 2007 Oct 5;131(1):146-59.
Note that there are some differences between the author's theoretical sequence and Addgene's quality control sequence. The differences do not appear to be in critical regions required for plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CTV was a gift from Klaus Rajewsky (Addgene plasmid # 15912 ; http://n2t.net/addgene:15912 ; RRID:Addgene_15912)
For your References section:Regulation of the germinal center response by microRNA-155. Thai TH, Calado DP, Casola S, Ansel KM, Xiao C, Xue Y, Murphy A, Frendewey D, Valenzuela D, Kutok JL, Schmidt-Supprian M, Rajewsky N, Yancopoulos G, Rao A, Rajewsky K. Science. 2007 Apr 27. 316(5824):604-8. 10.1126/science.1141229 PubMed 17463289