Addgene - pTAT-CRE Plasmid Data

Plasmid 35619: pTAT-CRE

Gene/insert name:
CRE
Species:
Bacteriophage P1
Entrez Gene:
cre (P1_gp003)
Fusion protein or tag:
TAT
Terminal:
N terminal on backbone
Fusion protein or tag:
T7 tag
Terminal:
N terminal on backbone
Fusion protein or tag:
6xHIS
Terminal:
C terminal on backbone
Vector backbone:
pET28
(Search Vector Database)
Backbone manufacturer:
EMD biosciences
Vector type:
Bacterial Expression
Promoter:
T7
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
T7 fwd List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Supplemental document:
pTAT-CRE.pdf (application/pdf)
Principal Investigator:
Steven Dowdy
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion proteins after lipid raft macropinocytosis. Wadia et al (Nat Med. 2004 Mar;10(3):310-5. Epub 2004 Feb 8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35619" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

35621: pTAT2.2

13763: pTriEx-HTNC

35432: hAAVS1 1R TALEN

35620: pTAT2.1

35612: pTAT-HA