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pTAT-CRE
(Plasmid #35619)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    CRE
  • Species
    Bacteriophage P1
  • Entrez Gene
    cre (a.k.a. P1_gp003)
  • Promoter T7
  • Tags / Fusion Proteins
    • TAT (N terminal on backbone)
    • T7 tag (N terminal on backbone)
    • 6xHIS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7 fwd
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTAT-CRE was a gift from Steven Dowdy (Addgene plasmid # 35619)
  • For your References section:

    Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion proteins after lipid raft macropinocytosis. Wadia JS, Stan RV, Dowdy SF. Nat Med. 2004 Mar;10(3):310-5. Epub 2004 Feb 8. 10.1038/nm996 PubMed 14770178