Addgene - pTAT2.1 Plasmid Data

Plasmid 35620: pTAT2.1

Gene/insert name:
None
Vector backbone:
pET28b
(Search Vector Database)
Backbone manufacturer:
EMD biosciences
Vector type:
Bacterial Expression
Backbone size (bp):
5368
Modifications to Backbone:
TAT domain (RKKRRQRRR) added between 6xHIS and MCS (BamHI-EcoRI-SacI-SalI-HindIII-NotI-XhoI). See attached map for cloning information.
Promoter:
T7
Fusion protein or tag:
6xHIS
Terminal:
N terminal on backbone
Fusion protein or tag:
TAT
Terminal:
N terminal on backbone
Fusion protein or tag:
6xHIS
Terminal:
C terminal on backbone
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Steven Dowdy
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion proteins after lipid raft macropinocytosis. Wadia et al (Nat Med. 2004 Mar;10(3):310-5. Epub 2004 Feb 8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35620" in your Materials and Methods section.

Price: $65.00

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