Addgene - pTAT-HA Plasmid Data

Plasmid 35612: pTAT-HA

Gene/insert name:
None
Vector backbone:
pREST-A
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Bacterial Expression
Backbone size (bp):
3000
Modifications to Backbone:
TAT domain (YGRKKRRQRRR) with 5' 6xHIS and 3' HA followed by MCS consisting of NcoI-KpnI-AgeI-XhoI-SphI-EcoRI-BstBI. See attached map for full cloning information.
Promoter:
T7
Fusion protein or tag:
6xHIS
Terminal:
N terminal on backbone
Fusion protein or tag:
TAT
Terminal:
N terminal on backbone
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Steven Dowdy
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Transduction of full-length TAT fusion proteins into mammalian cells: TAT-p27Kip1 induces cell migration. Nagahara et al (Nat Med. 1998 Dec;4(12):1449-52. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35612" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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