pa-G-GECO1.2
(Plasmid
#67622)
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Purposephotoactivatable fluorescent calcium reporter
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Depositing Lab
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Depositing OrganizationUniversity of California, Berkeley
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67622 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 67622-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 67622-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCMV
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Backbone manufacturerCampbell RE
- Backbone size w/o insert (bp) 3200
- Total vector size (bp) 4457
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Modifications to backboneCustomized Vector, see Addgene # 32446, Zhao et al Science. 2011.
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepa-G-GECO1.2
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Alt namephotoactivatable G-GECO1.2
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SpeciesSynthetic
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Insert Size (bp)1257
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MutationV81H, M84K, L189F, T190S
Resource Information
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A portion of this plasmid was derived from a plasmid made byclone purchased from Addgene, engineered by Robert Campbell. Publication: Zhao et al Science. 2011 Sep 8.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 67622-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 67622-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pa-G-GECO1.2 was a gift from Ehud Isacoff (Addgene plasmid # 67622 ; http://n2t.net/addgene:67622 ; RRID:Addgene_67622) -
For your References section:
Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging. Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, Rockwell NC, Martin SS, Lagarias JC, Isacoff EY. Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480. 10.1038/nmeth.3480 PubMed 26167640