Codon Optimized Dysferlin
PurposeThe human dysferlin cDNA was codon optimized in an attempt to increase Dysferlin production in human cells.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67878||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2940
- Total vector size (bp) 9180
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namecodon optimized Dysferlin
Insert Size (bp)6240
- Promoter No Promoter
/ Fusion Protein
- Cloning method Unknown
- 5′ sequencing primer GGT ACCTCGCGAA TGCATC
- 3′ sequencing primer CATATGGCGG CCGCGTCGAC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Codon Optimized Dysferlin was a gift from Matthew Hirsch (Addgene plasmid # 67878 ; http://n2t.net/addgene:67878 ; RRID:Addgene_67878)
For your References section:Oversized AAV transductifon is mediated via a DNA-PKcs-independent, Rad51C-dependent repair pathway. Hirsch ML, Li C, Bellon I, Yin C, Chavala S, Pryadkina M, Richard I, Samulski RJ. Mol Ther. 2013 Dec;21(12):2205-16. doi: 10.1038/mt.2013.184. Epub 2013 Aug 13. 10.1038/mt.2013.184 PubMed 23939025