|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22443||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5467
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)6057
- Promoter CMV
/ Fusion Protein
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Please note that the myoferlin insert in this plasmid contains K110R, K605R and I1821V mutations when compared to GenBank reference sequence AF182316. It is not known if these are polymorphisms or PCR artifacts.
The source of the human myoferlin gene used in this plasmid is described in the following publication: https://www.ncbi.nlm.nih.gov/pubmed/17702744
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDNA3.1-Myoferlin HA was a gift from William Sessa (Addgene plasmid # 22443 ; http://n2t.net/addgene:22443 ; RRID:Addgene_22443)
For your References section:Myoferlin regulates vascular endothelial growth factor receptor-2 stability and function. Bernatchez PN, Acevedo L, Fernandez-Hernando C, Murata T, Chalouni C, Kim J, Erdjument-Bromage H, Shah V, Gratton JP, McNally EM, Tempst P, Sessa WC. J Biol Chem. 2007 Oct 19. 282(42):30745-53. 10.1074/jbc.M704798200 PubMed 17702744