pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS
(Plasmid #78251)

• Purpose: Can be used to subclone in tagged gene of interest into inducible motor neuron expression vector.
• Depositing Lab: Nicholas Cole
• Depositing Organization: Macquarie University
  Located in Australia
• Publication: Don et al Zebrafish. 2016 Sep 15.

Backbone

• Vector backbone: pTol2pA2
• Backbone manufacturer: Chien Lab
• Backbone size w/o insert (bp): 4097
• Total vector size (bp): 8906
• Vector type: Zebrafish motor neuron inducible expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: -3mnx1
• Alt name: mnx1, Hb9
• Species: D. rerio (zebrafish)
• Insert Size (bp): 4809
• Entrez Gene: mnx1 (a.k.a. hlxb, hlxb9, zgc:112174)
• Promoter: -3mnx1

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Tol2-F [5'-CAATCCTGCAGTGCTGAAAA-3']
• 3′ sequencing primer: pBluescript-SK [5'-tctagaactagtggatc-3']

Gene/Insert 2

• Gene/Insert name: -loxP-mTagBFP2-stop-

Gene/Insert 3

• Gene/Insert name: mCherry-NS-MCS

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

DNA (Catalog # 78251-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 78251-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.