Addgene - FUW-OSKM Plasmid Data

Plasmid 20328: FUW-OSKM

Gene/insert name:
Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc
Insert size:
5000
Species:
M. musculus (mouse)
Vector backbone:
FUW (Lenti-Lox-Ubi)
(Search Vector Database)
Backbone manufacturer:
homemade
Vector type:
Lentiviral
Backbone size w/o insert (bp):
7222
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
ACTTTGCAGCCTGAGGGCCA List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
TOP10, 37C
High or low copy:
High Copy
Sequence: View sequences (4)
Principal Investigator:
Rudolf Jaenisch
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Reprogramming of murine and human somatic cells using a single polycistronic vector. Carey et al (Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20328" in your Materials and Methods section.