Addgene - TetO-FUW-OSKM Plasmid Data

Plasmid 20321: TetO-FUW-OSKM

Gene/insert name:
Oct-4
Alt name:
Sox2
Alt name:
Klf4
Alt name:
Myc
Insert size:
5000
Species:
M. musculus (mouse)
Entrez Gene:
Pou5f1 (NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
Sox2 (AL606746.1, Sox-2, lcc, ysb)
Klf4 (RP23-322L22.2, EZF, Gklf, Zie)
Myc (AU016757, Myc2, Niard, Nird, bHLHe39)
Vector backbone:
FUW
(Search Vector Database)
Backbone manufacturer:
homemade
Vector type:
Lentiviral
Backbone size w/o insert (bp):
8376
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
ACTTTGCAGCCTGAGGGCCA List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
TOP10, 37C
High or low copy:
High Copy
Selectable markers:
Zeocin
Sequence: View sequences (5)
Map:
View map
Supplemental document:
Notes from Addgene (2)
Principal Investigator:
Rudolf Jaenisch
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Comments:

Please note that this plasmid has an extra XhoI site upstream of the TRE element. You can find the additional XhoI site in Addgene's sequence with mOct4-R primer. Please view Addgene's test digest with XhoI for the correct band pattern.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Reprogramming of murine and human somatic cells using a single polycistronic vector. Carey et al (Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20321" in your Materials and Methods section.