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pCDNA-3xHA-hTERT
(Plasmid #51637)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51637 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen/LIfe Technologies
  • Backbone size w/o insert (bp) 5500
  • Modifications to backbone
    N terminal 3xFLAG tag after Kozak
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TERT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3399
  • Entrez Gene
    TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xHA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer CMV/T7
  • 3′ sequencing primer bGH reverse
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Addgene full plasmid sequencing noted a conserved E286D substitution in hTert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA-3xHA-hTERT was a gift from Steven Artandi (Addgene plasmid # 51637 ; http://n2t.net/addgene:51637 ; RRID:Addgene_51637)
  • For your References section:

    Identification of ATPases pontin and reptin as telomerase components essential for holoenzyme assembly. Venteicher AS, Meng Z, Mason PJ, Veenstra TD, Artandi SE. Cell. 2008 Mar 21;132(6):945-57. doi: 10.1016/j.cell.2008.01.019. 10.1016/j.cell.2008.01.019 PubMed 18358808