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SV40 1: pBSSVD2005
(Plasmid #21826)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21826 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SV40 Large T
  • Alt name
    Simian virus-40 large-T antigen
  • Species
    simian virus
  • Insert Size (bp)
    2800
  • Mutation
    likely expresses aa109-708 of the large T antigen
  • Entrez Gene
    SV40gp6 (a.k.a. SV40gp6)

Cloning Information

Resource Information

Depositor Comments

SV40 large T antigen for immortalizing cells. See Author's Map for more information.

Please note that this plasmid likely expreses a truncated SV40 large T antigen protein starting with the methionine at amino acid 109, when compared to GenBank reference sequence YP_003708382.1 (see NC_001669.1 for the corresponding nucleotide sequence). The plasmid functions as described to immortalize MEFs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SV40 1: pBSSVD2005 was a gift from David Ron (Addgene plasmid # 21826 ; http://n2t.net/addgene:21826 ; RRID:Addgene_21826)