SV40 1: pBSSVD2005
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21826||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3200
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameSV40 Large T
Alt nameSimian virus-40 large-T antigen
Insert Size (bp)2800
Mutationlikely expresses aa109-708 of the large T antigen
Entrez GeneSV40gp6 (a.k.a. SV40gp6)
- Cloning method Unknown
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
SV40 large T antigen for immortalizing cells. See Author's Map for more information.
Please note that this plasmid likely expreses a truncated SV40 large T antigen protein starting with the methionine at amino acid 109, when compared to GenBank reference sequence YP_003708382.1 (see NC_001669.1 for the corresponding nucleotide sequence). The plasmid functions as described to immortalize MEFs.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SV40 1: pBSSVD2005 was a gift from David Ron (Addgene plasmid # 21826 ; http://n2t.net/addgene:21826 ; RRID:Addgene_21826)