Addgene - pBABE-puro SV40 LT Plasmid Data

Plasmid 13970: pBABE-puro SV40 LT

Gene/insert name:
SV40 LT
Alt name:
Simian virus-40 large-T antigen
Insert size:
2200
Species:
H. sapiens (human)
GenBank ID:
AF316141
Entrez Gene:
SV40gp6 (SV40gp6)
Vector backbone:
pBABE-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
Cloning site 5':
BamHI?
Site destroyed during cloning:
Unknown
Cloning site 3':
BamHI?
Site destroyed during cloning:
Unknown
5' sequencing primer:
pBABE 5' List of Sequencing Primers
3' sequencing primer:
pBABE 3'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (3)
Principal Investigator:
Thomas Roberts
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Human mammary epithelial cell transformation through the activation of phosphatidylinositol 3-kinase. Zhao et al (Cancer Cell. 2003 May . 3(5):483-95. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13970" in your Materials and Methods section.