Addgene - pBABE-puro-hTERT Plasmid Data

Plasmid 1771: pBABE-puro-hTERT

Gene/insert name:
hTERT
Alt name:
telomerase reverse transcriptase
Insert size:
3500
Species:
H. sapiens (human)
Entrez Gene:
TERT (CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
Vector backbone:
pBABE-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE 5' List of Sequencing Primers
3' sequencing primer:
pBABE-3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (4)
Map:
View map
Map:
View map
Map:
View map
Supplemental document:
pBABE protocol (application/pdf)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

Please note that there is a point mutation at base 1547, from A to G. This changes a glycine to an aspartate. Thus far it appears to behave like wild-type, but extensive biochemistry has not been performed.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization. Counter et al (Proc Natl Acad Sci U S A 1998 Dec 8;95(25):14723-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1771" in your Materials and Methods section.