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pBABE-hygro-hTERT
(Plasmid #1773)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBABE-hygro
  • Backbone size w/o insert (bp) 5558
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    hTERT
  • Alt name
    telomerase reverse transcriptase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3500
  • Entrez Gene
    TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE-3
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-hygro-hTERT was a gift from Bob Weinberg (Addgene plasmid # 1773)
  • For your References section:

    Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization. Counter CM, Hahn WC, Wei W, Caddle SD, Beijersbergen RL, Lansdorp PM, Sedivy JM, Weinberg RA. Proc Natl Acad Sci U S A 1998 Dec 8;95(25):14723-8. 10.1073/pnas.95.25.14723 PubMed 9843956