1
Plasmid 1773: pBABE-hygro-hTERT
  • Retroviral expression of hTERT. Used to create immortalized cell lines.

  • hTERT

  • telomerase reverse transcriptase

  • 3500

  • H. sapiens (human)

  • TERT (CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)

  • pBABE-hygro
    (Search Vector Database)

  • Mammalian Expression, Retroviral

  • 5558

  • EcoRI

  • No

  • SalI

  • No

  • pBABE 5' List of Sequencing Primers

  • pBABE-3

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Hygromycin

  • View sequences (3)
  • View map

  • View map

  • View map

  • pBABE protocol (application/pdf)

  • Notes from Addgene (1)

  • Bob Weinberg

  • UBMTA

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization. Counter et al (Proc Natl Acad Sci U S A 1998 Dec 8;95(25):14723-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1773" in your Materials and Methods section.