|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17225||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6500
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Gene/Insert namePOU class 5 homeobox 1
SpeciesH. sapiens (human)
Insert Size (bp)1083
Entrez GenePOU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
Addgene has confirmed the presence of GFP in the pMIG vector backbone by sequencing, but the functionality of the GFP has not been confirmed.
Note: Visit www.addgene.org/18119 to find the pMIG-cMyc used in this paper
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMIG-hOCT4 was a gift from George Daley (Addgene plasmid # 17225 ; http://n2t.net/addgene:17225 ; RRID:Addgene_17225)
For your References section:Reprogramming of human somatic cells to pluripotency with defined factors. Park IH, Zhao R, West JA, Yabuuchi A, Huo H, Ince TA, Lerou PH, Lensch MW, Daley GQ. Nature. 2008 Jan 10. 451(7175):141-6. 10.1038/nature06534 PubMed 18157115